Hogeschool van Amsterdam

Centre of Applied Research Technology

Amino acid profiling from fingerprints

using Amide Stationary-Phase UPLC-MS


UPLC-MS is a commonly used technique to first separate complex samples and subsequently quantify molecules of interest. Herein we describe the use of UPLC-MS using an amide stationary phase to quantify non-derivatized amino acids extracted from fingerprints. As detector either a triple-quadrupole MS/MS or a TOF-MS detector was used. This method allows for a simple and fast sample preparation, which facilitates the analysis of large amounts of samples.

Reference van Helmond, W., & de Puit, M. (2019). Amino acid profiling from fingerprints: using Amide Stationary-Phase UPLC-MS. In M. A. Alterman (Ed.), Amino acid analysis: methods and protocols (2 ed., pp. 439-450). (Methods in molecular biology (Clifton, N.J.); Vol. 2030). New York: Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9639-1_33
Published by  Centre for Applied Research Technology 1 January 2019